Hifair™ V Reverse Transcriptase

Details:

Description

Hifair™ V Reverse Transcriptase is an updated version of HieffTM M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than HieffTM M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. HifairTM V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.

Features

  • High-temperature resistance: Suitable for RNA templates with complex secondary structures.
  • Suitable for reverse transcription of a small number of templates and low-copy genes.

Applications

  • Quantification of fluorescence
  • Cloning of genes

Specifications

Size (Final Product) 10kb
Optimum reaction temperature 42℃
PCR method RT-PCR
Reverse Transcriptase M-MLV
Type of sample RNA
Ribonuclease H activity Low activity
Product type cDNA

Components

Components No. Name 11300ES92 (10000U) 11300ES93 (5×10000U)
11300-A Hifair™ V Reverse Transcriptase (200U/μL) 50 μL 5×50 μL
11300-B 5×HifairTM V Buffer 250 μL 5×250 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years.

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