The Hieff UniconTM nCov Multiplex One Step RT-qPCR Probe Kit is a kit for performing multiplex quantitative PCR reactions using RNA as template. During the experiment, reverse transcription and quantitative PCR are performed in the same reaction tube, simplifying the experiment and reducing the risk of contamination.
The kit uses heat-resistant HifairTM V Reverse Transcriptase for efficient synthesis of first strand cDNA and UNICONTM HotStart Taq DNA Polymerase for quantitative amplification. The kit mainly contains optimized MP Buffer, Enzymes Mix, etc. The MP buffer already contains Mg2+ and dNTPs, etc. Factors that effectively inhibit non-specific amplification and factors that enhance the amplification efficiency of multiplex qPCR reactions have been added, enabling up to four reactions to be performed while ensuring primer amplification efficiency.
Equipment with Rox: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus
Equipment with low Rox: ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6,7,12k Flex; Stratagene MX3000P, MX3005P, MX4000P
Equipment without Rox:
Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4
Eppendorf Mastercycler ep realplex, realplex 2 s; Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000
Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96
Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR
The product is shipped with ice packs and can be stored at -20°C for 2 years. Please avoid repeated freeze-thaw. Recommend storage in separate packs.
1. Please use RNase free consumables during the experiment.
2. For your safety and health, please wear lab coats and disposable gloves for operation.