Product Description

The Hieff Unicon^TM nCov Multiplex One Step RT-qPCR Probe Kit is a kit for performing multiplex quantitative PCR reactions using RNA as template. During the experiment, reverse transcription and quantitative PCR are performed in the same reaction tube, simplifying the experiment and reducing the risk of contamination.

The kit uses heat-resistant HifairTM V Reverse Transcriptase for efficient synthesis of first strand cDNA and UNICON^TM HotStart Taq DNA Polymerase for quantitative amplification. The kit mainly contains optimized MP Buffer, Enzymes Mix, etc. The MP buffer already contains Mg²⁺ and dNTPs, etc. Factors that effectively inhibit non-specific amplification and factors that enhance the amplification efficiency of multiplex qPCR reactions have been added, enabling up to four reactions to be performed while ensuring primer amplification efficiency.

Applicable Models

Equipment with Rox: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus

Equipment with low Rox: ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6,7,12k Flex; Stratagene MX3000P, MX3005P, MX4000P

Equipment without Rox:

Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4

Eppendorf Mastercycler ep realplex, realplex 2 s; Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000

Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96

Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for 2 years. Please avoid repeated freeze-thaw. Recommend storage in separate packs.

Cautions

1. Please use RNase free consumables during the experiment.

2. For your safety and health, please wear lab coats and disposable gloves for operation.