Pseudouridine-5-triphosphate solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.
|CAS No.||1175-34-4 (free acid)|
|Molecular formula||C9H15N2O15P3 (free acid)|
|Molecular weight||484.14 g/mol (free acid)|
|Content||100 mM ± 3 mM|
|10656||Pseudo UTP Tris Solution GMP-grade (100 mM)||20 μL||100 μL||1 mL||5 mL|
The product should be stored at -25℃ ~ -15℃ for two years.
Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.
Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by Dot Blot method.