Organoids are three-dimensional cell aggregates derived from organ progenitor cells, which can differentiate and self-organize to form part of the specific functions and structures of corresponding organs in the human body. The growth of organoids mainly depends on the support of various cytokines and the three-dimensional environment provided by basement membrane matrix. The categories of these cytokines mainly include: activators, inhibitors and hormones that promote cell growth and differentiation-related pathways. Organoids can be widely used in research fields such as drug development, disease modeling, cancer research, and developmental biology.