Hieff UNICON™ Hotstart High Specific Taq DNA Polymerase, 5 U/μL
Hieff UNICON™ Hotstart High Specific Taq DNA Polymerase is a double blocked HotStart DNA polymerase by using the company’s self-developed double antibody. This antibody can inhibit the activity of 5’ → 3’ polymerase activity, and 5’ → 3’ exonuclease activity. Heating for 30 sec at a pre-denaturing temperature, the antibody is completely inactivated, and the DNA polymerase and exonuclease activity are released. Such double antibody-mediated Hot-Start capability can effectively inhibit the non-specific amplification caused by mismatch or primer dimer, and the decrease of fluorescence signal generated by probe degradation which makes the in vitro detection reagent more stable during transportation or using at room temperature.
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Product Information
Product name | Cat# | Size |
10726ES72 | 250 U | |
10726ES76 | 500 U | |
Hieff UNICONTM Hotstart High Specific Taq DNA Polymerase, 5 U/μL | 10726ES80 | 1000 U |
10726ES92 | 10000 U | |
10726ES93 | 25000 U |
Product Description
Hieff UNICONTM Hotstart High Specific Taq DNA Polymerase is a double blocked HotStart DNA polymerase by using the company’s self-developed double antibody. This antibody can inhibit the activity of 5’ → 3’ polymerase activity, and 5’ → 3’ exonuclease activity. Heating for 30 sec at a pre-denaturing temperature, the antibody is completely inactivated, and the DNA polymerase and exonuclease activity are released. Such double antibody-mediated Hot-Start capability can effectively inhibit the non-specific amplification caused by mismatch or primer dimer, and the decrease of fluorescence signal generated by probe degradation which makes the in vitro detection reagent more stable during transportation or using at room temperature.
Package Information
Component number | Components | Cat# /Size | ||||
10726ES72 (250 U) | 10726ES76 (500 U) | 10726ES80 (1000 U) | 10726ES92 (1000 U) | 10726ES93 (25000 U) | ||
10726 | Hotstart High Specific Taq | 50 μL | 100 μL | 200 μL | 2 mL | 5 mL |
Shipping and Storage
The product is shipped with ice packs plus dry ice and can be stored at -20°C for 2 years.
Reaction System
Components | Volume (μL) | Final concentration |
2×Buffer a | 25 | 1× |
Primer/Probe mix b | X | 0.1 μΜ-0.5 μmol/L |
Hotstart High Specific Taq (5 U/μL) | 1.2 | 0.12 U/μL |
DNA template c | X | 0.1-100 ng |
ddH2O | up to 50 | - |
[Note]: a) According to the specific experimental application, it is recommended to prepare the corresponding reaction buffer.
b/c) The DNA amount and primer concentration in the table above are the recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.
Amplification Procedure (two-step method)
Cycle step | Temperature | Time | Cycles |
Initial denaturation | 5 min | 1 | |
Denaturation Annealing / Extension | 95℃ 60℃ a | 15 sec 30 sec b | 45 |
[Note]: a) Amplification reaction: the annealing temperature can be adjusted according to the Tm values of designed primers.
b) Fluorescent signal acquisition: the fluorescence signal acquisition time required by different qPCR instruments is different, please set it according to the shortest time limit.
