Hieff UNICON™ Hotstart High Specific Taq DNA Polymerase, 5 U/μL

Hieff UNICON™ Hotstart High Specific Taq DNA Polymerase is a double blocked HotStart DNA polymerase by using the company’s self-developed double antibody. This antibody can inhibit the activity of 5’ → 3’ polymerase activity, and 5’ → 3’ exonuclease activity. Heating for 30 sec at a pre-denaturing temperature, the antibody is completely inactivated, and the DNA polymerase and exonuclease activity are released. Such double antibody-mediated Hot-Start capability can effectively inhibit the non-specific amplification caused by mismatch or primer dimer, and the decrease of fluorescence signal generated by probe degradation which makes the in vitro detection reagent more stable during transportation or using at room temperature.

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Product Information

Product name

Cat#

Size

10726ES72

250 U

10726ES76

500 U

Hieff UNICONTM Hotstart High Specific Taq DNA Polymerase, 5 U/μL

10726ES80

1000 U

10726ES92

10000 U

10726ES93

25000 U

Product Description

Hieff UNICONTM Hotstart High Specific Taq DNA Polymerase is a double blocked HotStart DNA polymerase by using the company’s self-developed double antibody. This antibody can inhibit the activity of 5’ → 3’ polymerase activity, and 5’ → 3’ exonuclease activity. Heating for 30 sec at a pre-denaturing temperature, the antibody is completely inactivated, and the DNA polymerase and exonuclease activity are released. Such double antibody-mediated Hot-Start capability can effectively inhibit the non-specific amplification caused by mismatch or primer dimer, and the decrease of fluorescence signal generated by probe degradation which makes the in vitro detection reagent more stable during transportation or using at room temperature.

 Package Information

Component number

Components

Cat# /Size

10726ES72

(250 U)

10726ES76

(500 U)

10726ES80

(1000 U)

10726ES92

(1000 U)

10726ES93

(25000 U)

10726

Hotstart High Specific Taq

50 μL

100 μL

200 μL

2 mL

5 mL

Shipping and Storage

The product is shipped with ice packs plus dry ice and can be stored at -20°C for 2 years.

Reaction System

Components

Volume (μL)

Final concentration

2×Buffer a

25

Primer/Probe mix b

X

0.1 μΜ-0.5 μmol/L

Hotstart High Specific Taq (5 U/μL)

1.2

0.12 U/μL

DNA template c

X

0.1-100 ng

ddH2O

up to 50

-

[Note]: a) According to the specific experimental application, it is recommended to prepare the corresponding reaction buffer.

b/c) The DNA amount and primer concentration in the table above are the recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.

Amplification Procedure (two-step method)

Cycle step

Temperature

Time

Cycles

Initial denaturation 

95℃

5 min

1

Denaturation 

Annealing / Extension

95℃

60℃ a

15 sec

30 sec b

45

[Note]: a) Amplification reaction: the annealing temperature can be adjusted according to the Tm values of designed primers.

  b) Fluorescent signal acquisition: the fluorescence signal acquisition time required by different qPCR instruments is different, please set it according to the shortest time limit.