Hieff Unicon™ Hotstart Direct Taq DNA Polymerase, 5 U/μL

Hieff Unicon™ Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an antibody and has high amplification sensitivity and specificity. The antibody can be completely inactivated by heating for 30 seconds at a denaturation temperature and the activity of DNA polymerase is restored. Use of the "hot start" Taq DNA Polymerase can effectively inhibit the amplification caused by nonspecific annealing of PCR primers.

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Product Information

Product name

Cat#

Size

10717ES72

250 U

10717ES76

500 U

Hieff UniconTM Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES80

1000 U

10717ES92

10 KU

10717ES93

25 KU

10717ES94

50 KU

Product Description

Hieff UniconTM Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an antibody and has high amplification sensitivity and specificity. The antibody can be completely inactivated by heating for 30 seconds at a denaturation temperature and the activity of DNA polymerase is restored. Use of the "hot start" Taq DNA Polymerase can effectively inhibit the amplification caused by nonspecific annealing of PCR primers.

Product Component

Component Number

Component

Cat#/Size

10717ES72 (250 U)

10717ES76 (500 U)

10717ES80 (1000 U)

10717ES92 (10 KU)

10717ES93 (25 KU)

10717ES94 (50 KU)

10717

Hotstart D-Taq (5 U/μL)

50 μL

100 μL

200 μL

2 × 1 mL

5 × 1 mL

10 mL

Shipping and Storage

The Hieff UniconTM Hotstart Direct Taq DNA Polymerase products are shipped with ice pack and can be stored at -20°C for 2 years.

Reaction System

Components

Vloume(μL)

Final Concentration

2× Buffera

25

Primer/Probe mixb

X

0.1 μmol/L-0.5 μmol/L

Hotstart D-Taq (5 U/μL)c

1.2

0.12 U/μL

Template DNA

X

0.1-100 ng

Water, nuclease-free

up to 50

-

[Note]:

(a) According to the different experimental requirements, you need to prepare your own corresponding reaction Buffer. If basic reaction Buffer is required, Cat#11374 is recommended.

(b/d) The amount of DNA and primer concentration in the table above, both are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.

(c) Adjust the amount of Taq enzyme according to the specific experimental application.

Refer to The Amplification Procedure (Two-Step Protocol)

Cycle step

Temperature

Time

Cycles

Initial denaturation

95

5 min

1

Denaturation

Annealing / Extension

95

60a

15 sec

30 secb

45

[Note]:

a) Amplification reaction: The amplification reaction temperature is adjusted according to the designed primer Tm values.

b) Fluorescent signal acquisition: Different qPCR instruments require different fluorescence signal acquisition time, please set according to the minimum time limit.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. This product is for research use ONLY