Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody



Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a mixture of two monoclonal antibodies who can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease of Taq DNA Polymerase, respectively. The antibody can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification. In addition, the product can effectively prevent probe degradation. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibodies.



Hot-start PCR


Components No. Name 31303ES60 31303ES80 31303ES90 31303ES92 31303ES93 31303ES94
31303 Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody (5 mg/mL) 100 µg 1 mg 5 mg 10 mg 100 mg 1000 mg


Classification Monoclonal
Type Antibody
Antigen Taq DNA Polymerase
Form Liquid

The concentration of this product is 5 mg/mL. 1 μL of antibody could block the activity of 20-50 U of Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase at room temperature for 1 hour (incubate at room temperature for 2 hours when volume is greater than 200 mL, and customer should adjust the process when applied to larger volume), and then store at -20℃ overnight before use.

Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio needs to be adjusted appropriately to achieve that the blocking efficiency is better than 95%.

Shipping and Storage

The product is shipped with ice packs and can be stored at -25°C~-15℃ for 2 years.

Notes: Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!


Fig 1. Detection of blocking efficiency of double-block antibody for exonuclease activity; Yellow: unclosed Taq DNA polymerase group; Purple: Taq DNA polymerase group blocked by double-block antibody

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