Hieff™ Double-Block anti-Taq DNA Polymerase Antibody

Anti-Taq DNA Polymerase Antibody is a monoclonal antibody used for hot-start PCR technology. After binding to the Taq enzyme, it inhibits DNA polymerase activity at low temperatures. It can effectively inhibit the non-specific annealing of primers and the non-specific amplification caused by primer dimers under low-temperature conditions. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody from Yeasen not only can block the polymerase activity of Taq DNA polymerase, but also block the exonuclease activity of Taq DNA polymerase at the same time. This can effectively prevent non-specific amplification caused by mismatching or primer dimers, and also prevent material degradation to generate non-specific signals.

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Product Description

Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is a double blocking Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperature. In addition, the product can effectively prevent probe degradation. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibody.


Product Component

Component Number

Component

Cat# /Size

31303ES60(100 µg)

31303ES80

(1 mg)

31303ES90

(5 mg)

31303ES92(10 mg)

31303ES93(100 mg)

31303ES94

(1000 mg)

31303

Hieff™ Double-Block anti-Taq DNA Polymerase Antibody

20 μL

200 μL

1 mL

1 mL×2

20 mL

200 mL


Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for 2 years.


Applications

The concentration of this product is 5 mg/mL. 1 μL antibody could block the activity of 20-50 U Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase well and incubate at room temperature for 20 min prior to use. The blocking efficiency of 5 '- 3' polymerase and 5 '- 3' exonuclease activity is over 90%.